Key Publications

• Papers
• Patents
• Abstracts
• Research & Development Collaborative Projects and Achievements

Papers

Papers published in collaboration with the University of the West of England.

Pemberton, R. M., Xu, J., Pittson, R., Biddle, N., Drago, G. A., Jackson, S. K. and Hart, J.P. (2009).

Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture.
Analytical Biochemistry, 385, 334-341.

Pemberton, R. M., Pittson, R., Biddle, N. and Hart, J.P. (2009).

Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application on serum analysis.
Biosensors & Bioelectronics, 5. 1246-1252.

Hart, J.P., Serban, S., Jones, L.J., Biddle, N., Pittson, R., Drago, G.A. (2006).

Selective and rapid biosensor integrated into a commercial hand-held instrument for the measurement of ammonium ion in sewage effluent.
Analytical letters, 39, Pages: 1657-1667.

Pemberton, R.M., Pittson, R., Biddle, N., Drago, G.A., Hart, J.P. (2006).

Studies towards the development of a screen-printed carbon electrochemical immunosensor array for mycotoxins: A sensor for Aflatoxin B1.
Analytical Letters, 39, Pages: 1573-1586.

Pemberton, R.M., Pittson, R., Biddle, N., Drago, G.A., Lonsdale, D. J., Hart, J.P. (2006).

Towards a commercial instrument based on anummunosensor array for automated electrochemical detection of mycotoxins in grain.
57th Annual ISE Electrochemistry meeting, Edinburgh UK.

Pemberton, R.M., Pittson, R., Biddle, N., Drago, G.A., Lonsdale, D. J., Hart, J.P. (2006).

A screen-printed Carbon electrochemical immunosensor for the determination of Mycotoxins in grain.
9th World Congress on Biosensors, Toronto, Canada.

Crouch, E., Cowell, D.C., Hoskins, S., Pittson, R., Hart, J.P. (2005).

A Novel, Disposable, Screen Printed Biosensor for Glucose in Serum Fabricated using a Water-Based Carbon Ink.
Biosensors & Bioelectronics, 21, Pages: 712-718.

Crouch, E., Cowell, D.C., Hoskins, S., Pittson, R., Hart, J.P. (2005).

Amperometric, screen-printed, glucose biosensor for analysis of human plasma oxidase using a biocomposite water-based carbon ink incorporating glucose oxidase.
Analytical Biochemistry, 14, Pages: 17-23.

Boujtita, M., Hart, J.P., and Pittson, R. (2000).

Development of a Disposable Ethanol Biosensor Based on a Chemically Modified Screen-printed Carbon Electrode Coated with Alcohol Oxidase for the Analysis of Beer.
Biosensors and Bioelectronics, 15, Pages: 257-263.

 

Papers published in collaboration with the University of Leeds.

Vakurov, A., Simpson, C. E., Daly, C. L., Gibson, T., Millner, P.A. (2005)

Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection. II. Immobilisation and stabilisation of acetylcholinesterase.
Biosensors and Bioelectronics, 20, Pages: 2324-2329.

Azevedo, A. M., Cabral, J. M. S., Gibson, T., Fonseca, L. P. (2004)

Operation and performance of analytical packed-bed reactors with an immobilised alcohol oxidase.
Journal of Molecular Catalysis B: Enzymatic, 28, Pages: 45-53.

Azevedo, A. M., Cabral, J. M. S., Prazers, D. M. F., Gibson, T., Fonseca, L. P. (2004)

Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase.
Journal of Molecular Catalysis B: Enzymatic, 27, Pages: 37-45.

Vakurov, A., Simpson, C. E., Daly, C. L., Gibson, T., Millner, P.A. (2004)

Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection. I. Modification of carbon surface for immobilization of acetylcholinesterase.
Biosensors and Bioelectronics, 20, Pages: 1118-1125.

Grant, S., Davis, F., Prichard, J. A., Law, K. A., Higson, S. P. J., Gibson, T. (2003)

Labeless and reversible immunosensor assay based upon an electrochemical current-transient protocol.
Analytical Chimica Acta, 495, Pages: 21-32.

Drago, G. A., Gibson, T. (2001)

Enzyme Stability. Applications and Case Studies
Engineering and Manufacturing for bioelectronics, 15, Pages: 361-376.

Drago, G. A., Gibson, T. (2001)

Enzyme stability and stabilisation: Applications and case studies.
In “Engineering and Manufacturing for Biotechnology” Eds. Hofman, M. & Thonart, P. pp.361-376. Kluwer Academic publishers.

Appleton, B. & Gibson, T.D. (2000).

Detection of total sugar concentration using photoinduced electron transfer materials: development of operationally stable, reusable optical sensors.
Sensors and Actuators, B. Chem. 65, 302-304.

Korpan, Y. I., Dzyadevich, S. V., Arkhipova, A. N., Elskaya, A. V., Gonchar, M. V., Gibson, T. D., Jaffrezic-Renault, N/. Martelet, C., Soldatkin, A. P. (2000).

Enzyme- based electrochemical sensor formaldehyde detection.
Sensors and Materials. 12, 79-87.

Korpan, Y. I., Gonchar, M. V., Sibirny, A. A., Martelet, C., Elskaya, A. V., Gibson, T. D., Sldatkin, A. P. (2000).

Development of highly selective and stable potnetiometric sensors for foemaldehyde determination.
Biosensors and Bioelectronics, 15, Pages: 77-83.

Hussain, M., Drago, G.A., Colyer, J. & Orchard, C.H. (1999).

The effect of protein kinase A inhibitor H89 on Ca²⁺ regulation in isolated ferret cardiac ventricular myocytes.
Pflugers. Arch. 437, No:4, 529-537

Levine, B.A., Patchell, V.B., Sharma, P., Gao, Y., Biglow, D.J., Yao, Q., Goh, S., Colyer, J., Drago, G.A. & Perry, S.V. (1999).

Sites on the cytoplasmic region of phospholamban involved in interaction with the calcium-activated ATPase of the sarcoplasmic reticulum.
Euro. J. Biochem. 264, 905-913.

Simons, H. & Gibson, T.D. (1999).

Rapid continuous colorimetric enzyme assay for penicillin G acylase.
Biotechnology Techniques 13, 365-367.

McAteer, K., Simpson, C.E., Gibson, T.D., Gueguen, S., Boujtita, M. & El Murr, N. (1999).

Proposed Model for Shelf Life Prediction of Stabilised Commercial Enzyme Based Systems and Biosensors.
J. Mol. Catal. B: Enzymatic. 7, 47 - 56.

Clements, J., Boden, N., Gibson, T. D., Chandler, R. C., Hulbert, J. N., RuckKeene, E. A. (1998)

Novel, self-organising materials for use in gas sensor arrays: beating the humidity problem.
Sensors and Actuators: B Chemical, 47, Page 37-42.

Chaniotakis, N.A., Gavalas, V.G. & Gibson, T.D. (1998).

Improved Operational Stability of Biosensors Based on Enzyme-Polyelectrolyte Complex Adsorbed Into a Porous Carbon Electrode.
Biosensors & Bioelectronics 13, 1205-1211.

Drago, G.A., Colyer, J. & Lederer, W.J. (1998).

Immunofluorescence localization of SERCA2a and the phosphorylated forms of phospholamban in the intact rat cardiac ventricular myocytes.
Ann. New. York. Acad. Sci. 853, 273-279.

Gibson, T.D., Hulbert, S. & Simpson, C.E. (1998).

Stabilised enzyme systems for use in the food industry.
The European Food & Drink Review pp.24-28

Gibson, T.D., Pierce, B. J. L., Parker, S. M. (1998).

Stabilisation of the biological components of biosensors.
Biosensors for Food Analysis: Page 46-53.

Gavalas, V. G., Bibson, T. D., Chaniotakis, N. A. (1998).

Improved operational stability of biosensors based enzyme-polyelectrolyte complex adsorbed into a porus carbon.
Biosensors and bioelectronics, 13, Page1205 - 1211.

Pierce, B. J. L., Gibson, T. D., Bunnel, P. A. (1998).

A preliminary model and evidence for the mechanism of stabilisation of analytical enzymes in aqueous solution by polyelectrolytes and sugar derivatives.
Biosensors for Food Analysis, pp. 54 - 60.

Woodward, J. R., Brunsman, A., Gibson, T. D., Parker, A. (1998).

Practical construction and function of biosensor systems for quality control in food and beverage industry.
Biosensors for Food Analysis, pp. 71-88.

Appleton, B., Gibson, T.D. & Woodward, J.R. (1997).

High Temperature Stabilisation of Immobilised Glucose Oxidase: Potential Applications in Biosensors.
Sensors and Actuators. B43, 65-69.

Gibson, T. D., Rippeth, J., Kibria, A. (1997).

Fabrication on shelf-stable biosensors: matrix engineering to preserve and orientate 3D enzyme structures.
Eurosensors XL. THe 11th European Conference on Solid State Transducers.

Gibson, T. D., Rippeth, J., Hulbert, S., Simpson, C. E., Appleton, B. (1997)

Enzyme stabilisation in biosensors using polyelectrolyte combinations.
Abstract of Papers of the America Chemical Society, 213, pp. 32.

Hussain, M., Drago, G.A., Colyer, J. & Orchard, C.H. (1997).

Rate dependent abbreviation of Ca²⁺ transient in rat heart is independent of phospholamban phosphorylation.
Am. J. Physiol. 437, No 4,529 - 537.

Korpan, Y I., Elskaya, A. V., Soldatkin, A. P., Gonchar, M. V., Sibirny, A. A., Gibson, T. D. (1997)

A novel enzyme biosensor specific for formaldehyde based on pH- sensitive field effect transistors.
Journal of Chemical Technology and biotechnology, 68, 209-213.

Rippeth, J.J., Gibson, T.D. (1997).

Stabilisation of acetylcholinesterase using charged polymers,: Towards shelf stable pesticide biosensors.
Abstracts of Papers of the America Chemical Society, 213, 368.

Rippeth, J.J., Gibson, T.D., Hart, J.P., Hartley, I.C. & Nelson, G. (1997).

Flow-Injection Detector Incorporating a Screen Printed Disposable Amperometric Biosensor for Monitoring Organophosphate Pesticides.
The Analyst. 122, 1425-1429.

Gibson, T.D. (1996).

Protein Stabilisation using Additives Based on Multiple Electrostatic Interactions. In ‘New Approaches to Stabilisation of Vaccines Potency’.
Dev. Biol. Stand. 87, 207-217. Ed. F Brown. Karger.

Gisbon, T. D., Pierce, B. J. L., Hulbert, J. N., Gillespie, S. (1996).

Improvements of the stability characteristics using protein-polyelectrolyte complexes.
Sensors and Actuators: B Chemical, 33, pp. 13-18.

Quirk, P.G., Patchell, V.B., Colyer, J., Drago, G.A. & Gao, Y. (1996).

Conformational effects of serine phosphorylation in phospholamban peptides.
Euro. J. Biochem. 236, 85-91.

Drago, G.A. & Colyer, J. (1994).

Discrimination between two sites of phosphorylation on adjacent amino acids by phosphorylation specific antibodies to phospholamban.
J. Biol. Chem. 269, 25073-25077.

Drago, G.A., Thomson, J.A. & Lincoln, P.J. (1994).

The degradation of a VNTR polymorphic site in various biological specimens when subjected to different temperature conditions.
Advances in Forensic Haemogenetics. Bar, W. Fiori, A. & Rossi, U. (eds). 5, pp. 271-274. Springer.

Gisbon, T. D., Pierce, B. J. L., Hulbert, J. N., Gillespie, S.

Improvements of the stability characteristics of biosensors using protein-polyelectrolyte complexes.
Submitted to Sensors and Actuators B. paper given at Transducers 95/Eurosensors IX Stockholm.

 

Other Papers

 

Diercks, S., Metfies, K., Medlin, L. K. (2008).

Development and adaptation of a multiprobe biosensor for the use in a semi-automated device for the detection of toxic algae.
Biosensors and Bioelectronics XX

Diercks, S., Metfies, K., Schroder, F,. Medlin, L. K., Colijn, F. (2008).

Detection of Phytoplankton with Nucleic acid sensors.
Alga Toxina: Nature, Occurrence, Effect and Detection, Springer Netherlands, 285-299.

Thomas, G. E., Bone, S., Drago, G. (2008).

Determination of Protein Denaturation and Glass Transition Temperature Using High-Frequency Time Domain Reflectometry
J. Phys. Chem. B, 112, Pages: 15903-15906.

Vojinovic, V., Bertin, L., Cabral, J. M. S., Fonseca, L. P. (2005)

Horseradish Peroxidase combined with Oxidase enzyme a valuable Bioanalytical Tool - Lactate Oxidase - A case study.
Engineering in Life Sciences, Volume 6, Issue 2, pgs 181 - 186

European Community Framework Programs (2001).

Case studies of Biotechnology Projects funded under Framework IV. Case study 2 “Directly interfaced and micro or nano structured detection”. Applied Enzyme Technology Ltd. Project Coordinator.
Published by BETA Technology Ltd in partnership with the DTI.

Levine, B. A., Patchell, V. B., Sharma, P., Gao, Y., Bigelow, D. J., Yao, Q., Goh, S., Coyler, J., Drago, G. A., Perry, S. V. (1999)

Sites on the cytoplasmic region of phospholamban involved in interaction with calcium-activated ATPase of the sarcoplasmic reticulum.
FEBS Journal, Volume, 264, No. 3, pp. 905-913.

Patents

Gibson, T.D., Pierce, B.L.J. & Webster, J.I. (1994).

Stabilisation of Proteins in Solution. International Patent Application. PCT/GB94/02180.

Gibson, T.D. & Woodward, J.R. (1991).

International patent application PCT/GB91/00443 Enzyme Stabilisation.

Gibson, T.D. & Woodward, J.R. (1989).

International patent PCT/GB89/01346 Enzyme Stabilisation.

Abstracts

 

 

 

Drago, G.A. & Gibson, T.G. (2000).

The electrophoretic analysis of protein-polyelectrolyte interactions as a measure of protein stability.
2^nd International Conference on Protein Stabilisation (Lisbon). P11.

Gibson, T.D. & McAteer, K. (1998).

Biosensor Stability, Towards Commercial Reality?
Proceedings of Biosensors ’98, Berlin, Germany, 3-5 June 1998, p.87.

Gibson, T.D. & Steele, D. (1998).

Molecular Dimensionality, Surface Chemistry and 3D Enzyme Structure Studies for Formation of Efficient Interfacial Connections between Transducers and Enzymes.
Proceedings of Eurosensors XII, Southampton, UK. 2, 805-808.

Gibson, T.D., Rippeth, J.J. & Hulbert, S. (1997).

Enzyme stabilisation in biosensors using polyelectrolyte combinations.
Abstr. Pap. Am. Chem. S 213, 31-bitec, Part 3.

 

 

   

Research & Development Collaborative Projects and Achievements.

 

 

EU Nanosecure 2008 - 2011

Development of detection and detoxification systems of airborne chemical and biological agents.

TSB Biomedical Ink Jet 2008 - 2009

Development of stabilisation systems for ink jettable enzyme, antibodies and cell formulation.

TSB Sensacell-tox 2008 - 2009

Development of multiple analyte electrochemical sensors for the continuous screening of pharmacological events in cell cultured cell lines using high through put platforms.

DEFRA Hortlink181 2007 - 2009.

Development of an electrochemical test for the detection of the sweetness of onions.

EU Spies Detox 2007 - 2009.

Development of an early warning system for the detection of algal toxins in shellfish. Involving toxin capture, detection and detoxification.

CBRN OP 2007 - 2008

The development of electrochemical sensors for the detection of organophosphate's.

DEFRA Mycotoxins 2005 - 2006.

The development of electrochemical sensors for the detection of mycotoxins in cereal.

DEFRA Hortlink164 2004 - 2007

Development of an electrochemical test for the detection of the sweetness of onions.

DTI Biowise Award STREAM 2003 - 2005.

Sensor Technology for Rapid Environmental Ammonia Monitoring.

DEFRA Rapid Analytical systems 2003 - 2004.

The development of electrochemical sensors for the detection of organophosphate's in cereal.

EU Safeguard 2001 - 2003

Development of a flow injection system for the detection of organophosphate's in water.

OP Link 1999 - 2001.

The development of electrochemical sensors for the detection of organophosphate's in water.

EU BIOMIST 1997 - 2000

Produce immobilised and stabilised enzymes for use in industrial bio-transformations.

EU Promofilm 1997 - 2000

The development of stabilised biomolecules for the incorporation into protein-containing single layer films.

EU Diamonds 1997 - 2000

The incorporation of biological systems for use in biosensor detection systems and their integration within novel instrumentation detection systems.

SMART Award Stage 2 1995

Enzyme Stabilisation.

SMART Award Stage 1 1994

Antibody Stabilisation.