Products
Protein Stabilisation Kits
The enzyme/protein stabilisation kit manufactured and marketed by Applied Enzyme Technology Ltd. is the result of 9 years of expertise in the field of protein stabilisation. The data supporting the composition of these kits has been accumulated from the combination of 2 Smart awards, 4 European Commission funded projects and a multitude of client contract research projects.
6 kits are currently manufactured by AET. One is a generic customised kit, which contains 23 formulations (STK0001) and stabilises proteins in the dry and solution states. The rest are smaller tailor-made kits containing 9 stabiliser formulations each. They have been designed to stabilise enzymes in the dry state (STKED), enzymes in solution (STKES), antibodies (STKAB) and one kit contains pharma-approved formulations (STKPH). A further kit has been added that provides a number of formulations required for freeze-drying proteins (STKFD). These formulations meet the majority of the stability issues encountered by clients working both in industry and academia. The flexibility of the kits allows the investigator to use their own buffer systems, pH, ionic strength etc. The formulations are made up in a concentrated form thus allowing for use with different buffer compositions and pH criteria. To date AET has stabilised over 50 enzymes/proteins in both liquid and dry forms.
AET currently distributes directly from the head office in the UK Gentaur a Benelux based distributor and the distributors of the Gwent Group Contacts
List of AET stabilisation kits
Applied Enzyme Technologies Protein Stabilisation Kit (product number
STK0001)
Stabilised Proteins Overview
The enzyme/protein groups studied and successfully stabilised are listed below:
Unconjugated polyclonal and monoclonal antibodies
Horseradish Peroxidase-Conjugated Antibodies
Alkaline Phosphatase conjugated antibodies
Esterases
Hydrolases
Oxidoreductases
Peroxidases
Luciferase
Kinases
Lipases
Phosphatases
Proteases
Oxidases
Most of the enzymes listed above have been stabilised individually. However, certain enzymes have been successfully combined to made stable working enzyme cocktails. Most contract research has led to the generation of stable enzyme formulations from between 50 days to over 18 months at temperatures of up to 50°C. The data generated by AET addresses both the issues of increased shelf life and the operational stability of the enzyme system in question.
By using our in-depth experience and knowledge, AET also offers a range
of stabilised enzyme systems as off-the-shelf products. These materials
are manufactured within a tightly controlled environment. Our commitment
to quality is highlighted by our adoption of ISO9001:2002 and TS13485
quality systems.
Most contract research has led to the generation of stable enzyme formulations from between 50 days to over 18 months at temperatures up to 50°C. The data generated by AET addresses both the issues of increased shelf life and the operational stability of the enzyme system in question.
Bio-Sensor Applications
In addition to protein stabilisation, AET also offers biosensor-manufacturing expertise, with liquid dispensing expertise and sensor fabrication knowledge. As we are preparing to launch our first biosensor system for detection of ammonia in river water together with our sister company Gwent Electronic Materials Ltd., we understand the difficulties that can be encountered with all aspects of biosensor fabrication and are therefore in a position to offer advice and consultancy services in this area.
Industrial Applications
Listed below are some of the examples in which our stabilisation technology is currently being used and can be used in the future.
- Biocatalysis Industry
- Bioremediation Industry
- Biosensor Industry
- Cosmetics Industry
- Diagnostics
- Human Healthcare
- Hygiene Industry
- Pharmaceutical Industry
For further information on pricing. Contact AET.
For further information on pricing. Contact AET.
Bulk Stabilisers
AET produces and supplies clients with bulk quantities of final stabiliser formulations. We have the production facilities to handle upto hundreds of litres or hundreds of kilos of stabiliser formulations.
For further information on pricing. Contact AET.
